Neuronal networks dynamics underlying spike timing precision during brain developmental plasticity and Alzheimer's disease progression.

Brain plasticity and neuronal network oscillations are crucial brain processes that depend on the timing precision of neuronal activity. These processes change during healthy brain development and are disrupted early on Alzheimers disease (AD) with controversies on the onset of their impairment. Thus, comprehensive studies of plasticity considering the state of the neuronal network during development and AD progression are missing. Here we will address the mechanisms underlying timing-dependent plasticity by the convergent study of spike timing-dependent plasticity and neuronal networks rhythms in mice hippocampal slices. The work plan includes logical sequential sets of experiments involving ex-vivo electrophysiology combined with multiphoton imaging, optogenetic, calcium dynamics, molecular biology, and immunohistochemistry during healthy development in wild-type mice and the progressive impairment of neuronal circuits dynamic in an AD mice model. The action will be implemented during 2 years in the Laboratory of Cellular Neuroscience and Plasticity (LCNP) lead by Professor Antonio Rodrguez Moreno (supervisor) and hosted by University Pablo de Olavide (UPO) in Seville, Spain. All the premises and infrastructures owned by UPO are at full disposal for both the candidate and the supervisor (Manager of the Infrastructure of Multiphoton Unit). During the action the researcher will acquire intellectual skills and learn sophisticated and cutting-edge approaches to assess synaptic plasticity supported by his previous skills and the strong supervisor's background. The expected outcomes from the insertion in a suitable scientific and academic environment at LCNP and UPO will lead the candidate to establish as a young PI within the Spanish Science System. LCNP and UPO will establish the study of neuronal dynamics in a highly convergent way and nurture from the candidate's expertise acquired in both the KI and during the fellowship.