Reading DNA in real time for medical applications

The aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, Laser-Assisted DNA Optical Mapping (LADOM), allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecules length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA. In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput bThe aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, Laser-Assisted DNA Optical Mapping (LADOM), allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecules length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA. In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput by developing a software to automate the data analysis.